FastQCFastQC Report
Sat 1 Jul 2023
H7TV5DRX3_n01_burcu-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TV5DRX3_n01_burcu-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98884
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGAGCAC7570.7655434650701832No Hit
GAAACTTAAAGGAATTGACGGAAGAGCACACGTCTGAACTCCAGTCACAC3300.3337243639011367TruSeq Adapter, Index 5 (96% over 33bp)
TCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTAGGGT1770.17899761336515513No Hit
GAAACTTATAGGAATTGACGGAAGAGCACACGTCTGAACTCCAGTCACAC1590.16079446624327495TruSeq Adapter, Index 5 (96% over 33bp)
TTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTAGG1480.14967032077990375No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG1460.1476477488774726No Hit
TGGGCGTAAAGCGTCTGTAGGTGGCTTTTTAAGTCCGCCGTCAAATCCCA1020.1031511670239877No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)