FastQCFastQC Report
Sat 24 Dec 2016
H7TTCBGX2_n02_brugia6-l3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TTCBGX2_n02_brugia6-l3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46737599
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG6338601.356210018405096No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG4939731.0569070952917372No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG4331300.9267271089385657No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA3102650.6638445419500476No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1745340.37343381717148116No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1612410.3449920480510777No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1589670.34012658630581344No Hit
GCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCA1459700.3123181402621902No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1397930.2991018002443814No Hit
AAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAAC1331170.2848177973370006No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1326980.2839213028465583No Hit
GTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTT1285060.2749520787321574No Hit
GCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG1279420.27374534151829233No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1237330.26473974411907636No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA1222830.261637316884849No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT1202760.2573431296716804No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG1164310.24911634848850495No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT1129410.2416491270764679No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA1101200.23561330140215378No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1089260.23305861304514167No Hit
GTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT1046730.2239588730264043No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA1020140.21826966336032794No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG1003930.2148013636729606No Hit
GTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACT998340.2136053244840412No Hit
GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA956770.20471098654425957No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG940670.2012662225117726No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT916240.19603916752334669No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT901340.19285115608955436No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA888060.19000976066399988No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT864440.18495601367969286No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT841250.17999426970991814No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC827420.17703519600996193No Hit
CGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTT819620.17536630411844648No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGCGGGGAAAGAA802150.17162841420244973No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC784520.16785629060662702No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT770210.1647945158671929No Hit
CCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATAT762440.16313204279064486No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA748140.1600724076561999No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC744870.15937275682475688No Hit
TGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGC742490.15886353083734575No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC741410.15863245349852054No Hit
GGAGTCTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGA734390.15713045079615665No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC725800.15529253011050054No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA713490.1526586763688909No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA706180.15109462512184246No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT703420.1505040941448447No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG700690.1499199819828143No Hit
GCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTAACT699100.14957978478954387No Hit
GCGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGAC688730.14736101441582397No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA687860.14717486878177033No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG682890.1461114851021765No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG669740.14329790454148061No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTA663010.14185795038380128No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG646040.13822704071726064No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA632850.13540490173660824No Hit
GCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAAAGCATAATGAA619310.1325078765813366No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC608340.13016072990826935No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG607890.13006444768375885No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT602590.128930457039524No Hit
CTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAAGT599570.12828429633280905No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG599570.12828429633280905No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA591470.12655121629161994No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC585070.12518186909858164No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC577640.12359214259166373No Hit
GTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATG571690.12231907762313592No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT570180.12199599726977846No Hit
GGCTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAA567570.12143756036761752No Hit
CCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTA559580.11972801598130875No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC550230.11772748531647935No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT549030.11747073271778467No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA531790.11378205371653771No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT521780.11164030912242624No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA518890.11102196328056989No Hit
GTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAGTTGA515370.11026882232439883No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACT515070.11020463417472516No Hit
CTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAG506700.10841378479882974No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT505650.10818912627497189No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC502750.1075686408281264No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA499800.10693745735633532No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA495680.1060559401008169No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA495020.10591472617153483No Hit
CCTCGTTCAAGGGAAATAATTGCAATTCCCGATCCCGATCACGGAGGATT484950.10376014394748861No Hit
CCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTAA483430.10343492398914203No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT477240.10211050850087529No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACT474150.10144937055923647No Hit
GTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT469430.10043947700437073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGC296850.083.7003564
CACCTGG302900.082.1705553
GGAGTCT145000.080.371751
CCTGGCC316250.078.6811145
GTTAGTT1218150.078.443751
GTCCAAT101800.077.921691
TCCAATC102900.076.6096952
CTTGTTA121150.076.408571
GAGTCTA153550.075.8601462
TTAGTTT1290350.074.165252
AGTTTTA1356350.070.997234
TAGTTTT1356300.070.664893
AGTCTAG165450.070.3219153
CTGGCCC401800.061.8421556
GGCCGAC26500.061.1168861
GTTACGA152850.060.6223074
TGGCCCG418450.059.339267
CTAGCAT331500.058.041681
GCCCGGA437500.056.9554149
CTGCAAG1377850.055.658991
Produced by FastQC (version 0.11.2)