FastQCFastQC Report
Sat 24 Dec 2016
H7TTCBGX2_n02_brugia5-l3d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TTCBGX2_n02_brugia5-l3d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64800584
Sequences flagged as poor quality0
Sequence length150
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG3837810.5922492920742813No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT3273670.5051914346944775No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA2499280.3856878820721739No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG2366800.3652436218784695No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC2334010.36018348229701136No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC2187240.3375339950639951No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1836470.28340331006893393No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC1818730.280665680420411No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA1563410.24126480094685565No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG1514380.23369851111218382No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1485090.22917849011978042No Hit
GCTAAATTGATTGGTTGAATACCGATAAGTTTTGTTAACTCTATCCGTTT1421420.2193529613868912No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC1402390.21641625945840243No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG1377760.2126153677874261No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT1365260.21068637282651648No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC1327270.20482377134132002No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1299290.20050590902081994No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1292150.19940406709914837No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT1274390.19666335105868799No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1226190.18922514648942054No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1223940.18887792739645679No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA1210430.1867930696427057No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA1210190.18675603293945622No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA1193870.18423753711849264No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1190310.1836881593536256No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1165310.17983016943180635No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA1138500.17569286103964743No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1135450.17522218626918548No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG1132460.1747607706745359No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1117040.1723811624907578No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC1108040.17099228611890288No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC1081370.16687658247030612No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG1052290.162388968593246No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC1045090.16127786749576206No Hit
CGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGC1040790.16061429322920917No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA1035120.15973930111494059No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA993740.1533535561963454No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG993720.15335046980440795No Hit
GCGGTGTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTT990130.1527964624516347No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA977400.15083197398344433No Hit
GTGGCTAGCAAGTTTACCTTGAAAAAATTAGAGTGCTCAATGCGGGCTAA973480.15022704116370308No Hit
CCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTC968710.14949093668661997No Hit
GCGTAACATAACCCCGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCG962420.14852026642229027No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC958650.14793848154207992No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT946970.146136028650606No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT927160.14307895743655644No Hit
GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC902230.1392317698865183No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG884180.13644630116296483No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG876120.13520248521217032No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA861110.13288614806311005No Hit
GTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACT856210.13212998203843349No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG853260.13167473922765882No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT840610.12972259632721828No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC837420.12923031681319416No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT834360.12875809884676348No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT808490.12476585087566494No Hit
ATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAAT806600.12447418683757541No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT796130.12285846065831751No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC790940.12205754195054785No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC786710.12140477005577605No Hit
GGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCT783180.12086002187881517No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC778550.12014552214529425No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT763470.11781838262445289No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG756840.11679524369718644No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC754510.11643567903647288No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT743790.1147813729579968No Hit
GTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGT740790.1143184141673785No Hit
CTGAAGTTTCCCTCAGGATAGCTGGAGTACGTTAAATAAAGCAGTTATAT734720.11338169421436078No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT733380.11317490595455126No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG718340.11085393921758482No Hit
GTCAGAATCGCTTCGGACTTTCACCAGAGTTTCCTCTGGCTTCATCCTGC717340.11069961962071205No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA704160.10866568733392896No Hit
GGGAAAACTGGCAATATTAGCCTAGGAGCCTAGTTCTCATCCAATGGATG700350.1080777296698437No Hit
GACGATATACACTTTGAGAGCTGGAATTACCGCGGCTGCTGGCACCAGAC697800.10768421469781815No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA687430.10608392047824754No Hit
GACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCTCGTT687160.10604225418709189No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA687100.10603299501127954No Hit
GTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTA684870.10568886231025325No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA684540.10563793684328525No Hit
GGCTAGCTCAGAGAGGACGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGC682360.1053015201221026No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC679100.10479843823629738No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC673330.1039080141623415No Hit
GTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT669540.1033231428901937No Hit
GCGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGAC665050.10263024790023498No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC659140.10171821908271692No Hit
GTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAACTAG654940.10107007677585128No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC651030.10046668715207877No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA649360.10020897342530122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCC640650.078.6922765
CACCTGG657650.076.8861853
ACCTGGC658450.076.793844
CTGGCCC739850.068.125566
TGGCCCG751100.067.172177
TAGTTTT912350.063.4475753
GCCCGGA810050.062.8463339
CTTGTTA173300.062.346351
GGAGTCT169950.060.9916341
GAGTCTA170300.060.959812
GTTAGTT951950.060.7954181
TGATTGG362400.059.018938
CTCCGAC57350.058.8665241
TTAGTTT993450.058.2281272
CGCCCTT43700.056.993281
AGTTTTA1038750.056.4971244
TTACCCT1030800.055.988398
TACCCTA1036450.055.7748879
CCAGACG183700.055.737326
GTCCAAT70700.054.0634881
Produced by FastQC (version 0.11.2)