FastQCFastQC Report
Sat 24 Dec 2016
H7TTCBGX2_n01_brugia6-l3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TTCBGX2_n01_brugia6-l3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46737599
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG6988351.4952308525733211No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG5001011.070018594665079No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG4266710.9129074003138244No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2973260.6361601929957934No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1700140.3637628026206481No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1637300.35031752486900325No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1466310.31373241916000005No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA1439080.30790627477461985No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1371310.29340617176333766No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1367280.29254391095272136No Hit
GCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCA1365130.29208389588006006No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1290050.27601974162172943No Hit
AAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAAC1286390.27523664619571064No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA1286210.2751981333059064No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG1282080.2743144764453989No Hit
GTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTT1260890.26978065347344865No Hit
GCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG1249890.26742708798541404No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT1156360.24741536252215265No Hit
CCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATAT1146990.24541055264734501No Hit
GTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACT1074100.2298149718816322No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA1073510.22968873518727392No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT1057970.22636378903417778No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT1046110.2238262175170787No Hit
GTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT1042100.22296823591644063No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG1007590.21558445909897941No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG975130.2086393013042882No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA960490.20550691960021308No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT959740.2053464492260289No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC954260.20417394569198985No Hit
GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA924090.19771875743980774No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT893940.19126784839760383No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT853660.18264951950141897TruSeq Adapter, Index 2 (97% over 37bp)
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT834800.1786142244919342No Hit
CGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTT808620.1730127386304119No Hit
CTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAG807500.1727731028716302No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC794310.1699509638909778No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT788360.16867789892245No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA777060.16626014528474176No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT775070.16583436389190637No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG764710.16361773312317562No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA755550.16165785495313956No Hit
TGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGC752040.1609068536019576No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG742740.1589170209620738No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGCGGGGAAAGAA738230.15795205911197963No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC716810.15336902522527954No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC701470.15008687117196584No Hit
GGAGTCTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGA691600.1479750810477021No Hit
GTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAGTTGA683930.14633400402104524No Hit
GCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTAACT672220.14382852657878295No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG665760.14244634175580992No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC664420.14215963468726753No Hit
GCGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGAC664010.1420719108827135No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA656120.14038376254629598No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG643460.13767502263006706No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT640140.13696467377367844No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG627680.134298725957232No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG621950.1330727322984649No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTA620690.13280314206983548No Hit
GCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAAAGCATAATGAA619640.13257848354597762No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT611650.13086893915966885No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT602990.1290160412390889No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA597130.1277622327154632No Hit
CTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAAGT591400.1265362390566961No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC589390.1261061784538825No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT585890.12535731670768965No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT584140.12498288583459327No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA583980.1249486521547673No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC577470.12355576930684865No Hit
GTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATG567000.12131560288323755No Hit
CCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTA553750.11848062627265042No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC551910.11808693895465192No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC541930.1159516131755078No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC540060.11555150704254191No Hit
CCTCGTTCAAGGGAAATAATTGCAATTCCCGATCCCGATCACGGAGGATT529870.11337124955862624No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACT529030.11319152273953996No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA514940.11017681930986657No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT514200.1100184885406715No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA511960.10953921702310809No Hit
GGCTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAA511100.10935521099404356No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA504830.10801367866586387No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG502940.10760929332291973No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA500870.1071663950901714No Hit
CCCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATA491350.10512949114052693No Hit
CGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTAT483760.10350553095378305No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA480580.10282513656724214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGC307750.087.142334
CACCTGG314850.085.08773
GTCCAAT99800.083.260231
GTTAGTT1286850.082.3537141
CCTGGCC330350.081.333055
CTTGTTA130500.079.343381
TCCAATC105350.078.7113952
TTAGTTT1356050.078.236972
AGTTTTA1376600.077.151654
TAGTTTT1391950.076.2198263
GGAGTCT143400.073.360691
GAGTCTA146850.071.662692
AGTCTAG152400.068.859953
GTTACGA156000.066.162054
GGCCGAC25950.064.651851
CTGGCCC421650.063.7049186
TTACGAC166250.062.039455
CAAAGGA134900.061.250638
GCCCGGA440450.061.0180559
CTAGCAT331700.060.4129561
Produced by FastQC (version 0.11.2)