FastQCFastQC Report
Sat 24 Dec 2016
H7TTCBGX2_n01_brugia4-l3d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TTCBGX2_n01_brugia4-l3d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53438840
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG6304761.1798085437483299No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC4943520.9250799605680063No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA4441960.8312231328374643No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG3983750.7454783823900369No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG3365630.6298097039531546No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC3086880.5776472692895279No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG2564190.47983638866412515No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC2361490.441905176085409No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG2150020.40233283506902473No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA2123780.39742254884275185No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC2074580.3882157621684902No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA2019800.37796479115190373No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA1860400.34813629936577967No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1788930.33476213181274145No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC1772060.33160525191040824No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1665530.3116703132029064No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA1647640.30832256089391163No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1639810.3068573344780688No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA1588410.2972388622208117No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC1572850.2943271223701712No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC1561010.29211150541441394No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT1543270.2887918225769871No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1411690.2641692821176508No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT1379590.2581624152021264No Hit
GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC1374240.2571612707162057No Hit
GTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTA1327290.24837552611546207No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT1245170.23300842608110503No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT1239160.2318837759202857No Hit
GTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATG1213590.22709886666701598No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA1201490.22483459596054103No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT1182950.22136520927475223No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1142020.21370598613293254No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG1087400.20348495588601848No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA1079760.20205528413416157No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT1042020.1949930050876853No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA1039900.19459628988952604No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT1035850.19383841415719355No Hit
GCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAA1026740.1921336615839715No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC1003940.18786710190565514No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC992190.18566832663283858No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT945750.17697801823542578No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT944880.17681521530033212No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT917810.1717496113313837No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTT902180.16882477239401156No Hit
GTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTT898470.16813052079723287No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA883470.16532357364044578No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTAC871310.16304807514534372No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT854910.15997914625392318No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT839230.1570449508260284No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG833830.15603444984958506No Hit
GTGGCTAGCAAGTTTACCTTGAAAAAATTAGAGTGCTCAATGCGGGCTAA829810.15528218801156612No Hit
CCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATC803100.15028395077438056No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC798080.1493445591259092No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT794100.14859978248030833No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA787150.14729923029766365No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG783650.14664427596107998No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT776740.1453512089708534No Hit
CTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGG776460.14529881262392672No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC774580.14494700858027607No Hit
GTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGA774090.14485531497315435No Hit
GTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAA773080.14466631386459736No Hit
CCCCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAA764070.1429802742724206No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC763140.1428062435486998No Hit
CCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAGG762960.14277256018281834No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG761080.1424207561391677No Hit
CCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATAT751300.14059062659294252No Hit
CGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAGAA750510.14044279404268506No Hit
CTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGGTC746790.13974667114780187No Hit
CCTCATTCTGAGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT742650.13897195373252863No Hit
GGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCT733660.13728965673656088No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA725870.13583191551313614No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG723880.13545952719033572No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC712980.13341981225640376No Hit
CCCACTTGGAGCACACATTCAATGTCTATGCTTCATAAAAAATTTAAGCA707120.13232323156715228No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT706410.13219036940173104No Hit
CTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCG695170.13008703033224525No Hit
ATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTA693610.12979510782793938No Hit
CTCCTCATTCTGAGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT685530.1282830989594834No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA678710.12700687365219754No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC677460.12677296138913194No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT673210.12597765969470895No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA673090.12595520411745464No Hit
CCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTC665090.12445816563383487No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT660140.12353187307209512No Hit
GTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTT654800.12253259988427893No Hit
CTCATTCTGAGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG648510.12135555337653287No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA647180.12110667072863109No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA647050.12108234385327227No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA629490.11779634438172686No Hit
CCCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAAT627140.11735658932716354No Hit
GTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT626630.11726115312383277No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT626250.11719004379586084TruSeq Adapter, Index 27 (97% over 39bp)
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA625570.11706279552475315No Hit
CGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTT618100.11566493584067318No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA617050.11546844953969808No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC610440.11423152149260725No Hit
GCCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAA592480.11087067009688086No Hit
CCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTA582830.1090648674260145No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACG580730.1086718948240643No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC580590.10864569665060095No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG577500.10806746553630282No Hit
GCGCGGTAACGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGA571480.10694094407737893No Hit
CTCTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGG566370.1059847107459668No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT558590.10452884082064655No Hit
TGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCA556080.10405914499641085No Hit
GCGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGAC549790.1028820984886648No Hit
CCTCCTCATTCTGAGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA548350.10261263156161324No Hit
GTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT544480.10188843919516216No Hit
GCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG541030.10124284134910115No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTTATTCTCCGTTACC539700.10099395870119936No Hit
AGGTGCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGG539490.10095466144100433No Hit
GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA538520.10077314552486544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTT1146650.084.995321
TAGTTTT1167100.083.555653
CCTGGCC310450.082.746065
TTAGTTT1200200.081.3225862
GCCTTGT927550.080.477341
AGTTTTA1272450.077.048324
ACCTGGC335400.076.8915564
CCAGACG130900.075.1659856
CACCTGG358600.072.0999153
GAGTCTA95550.070.213782
CCTTGTC1076600.069.690812
AGACAGG164550.068.9258961
AGTGTAG699700.067.3991555
CCAGTGT708650.066.499633
CTGGCCC391950.065.540296
TCCAGTG721400.065.1489942
GTAGCGC726200.064.988818
AGTCTAG88850.063.5136873
CAGACGT158800.062.050117
GGAGTCT108700.061.6765251
Produced by FastQC (version 0.11.2)