FastQCFastQC Report
Sat 24 Dec 2016
H7TTCBGX2_n01_brugia1-l3d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TTCBGX2_n01_brugia1-l3d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57208389
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG4582100.8009489657189962No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC3767500.6585572615932255No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA2302800.4025283774377915No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG2298900.40184665923733665No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2207710.3859066893143941No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC1932220.3377511644314962No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC1763930.3083341500841773No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC1644560.28746832916410214No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG1568910.2742447440706642No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC1549510.27085363302224785No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG1541110.2693853168981913No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA1462120.25557790134590225No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1461690.25550273754431363No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT1459700.2551548864625431No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT1454450.25423718888500774No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC1429160.24981650855436605No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA1304170.2279683142274816No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1279520.2236595056015299No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA1267680.2215898790647644No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1205080.2106474279497715No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA1200070.20977168226149492No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1074190.18776791634527587No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC1068640.18679777890616706No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT1064500.18607410881645348No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA1064390.18605488086720987No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT957930.16744572199017874No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA952150.16643538065719699No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA923990.16151302565083592No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG904930.15818134644553616No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC895630.15655571073675925No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG842220.1472196673812996No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT834530.14587545892963355No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA822150.14371144064203592No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA813260.14215747274407606No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT809100.1414303066635909No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA795510.13905478093431367No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG792960.13860904211093936No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA791640.1383783067200162No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT783130.136890762646716No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC781450.1365970994219047No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC735620.12858603656886755No Hit
GCGTAACATAACCCCGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCG733270.12817525765320886No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT724970.12672442148301014No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC724030.12656010991674665No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG713110.12465129895547312No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC711760.1244153195783926No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT711600.1243873516522201No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA691830.12093156477452982No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA681630.11914860948103259No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA670200.1171506507550842No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG665670.11635880884532511No Hit
GAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAG652130.11399202309297679No Hit
CCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATAT650330.11367738392353612No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG647500.1131827012293599No Hit
ATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAAT630290.11017440117042974No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA626720.10955036681770572No Hit
ACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCA620480.10845961769697797No Hit
GTGAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCA618180.10805757875824819No Hit
GCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAAT617310.10790550315968521No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT611250.10684621795590153No Hit
GTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAA609420.10652633480030349No Hit
CGCCGACGGAACGCTAGTTGGTATCGTTTACGGTCAGAACTAGGGCGGTA609160.10648088692027317No Hit
GGAGTCTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGA608990.10645117099871489No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG608080.10629210341860876No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC606240.10597047226762495No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT606000.1059285203783662No Hit
GTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT602270.10527651809946964No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC601670.10517163837632275No Hit
GGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTAC600430.10495488694848581No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT596140.10420499692798552No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA595820.10414906107564051No Hit
CCCACTTGGAGCACACATTCAATGTCTATGCTTCATAAAAAATTTAAGCA595540.10410011720483861No Hit
CCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAGG594450.10390958570778842No Hit
GTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTT589320.10301286407488244No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA588250.10282582856860382No Hit
CTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAG583770.10204272663577364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCC444450.076.826875
ACCTGGC478200.071.705874
CACCTGG508250.067.5249563
GTTAGTT1077600.065.968291
GAGTCTA147550.065.76272
CTGGCCC520700.065.604186
TAGTTTT1085350.065.529463
GCCCGGA541200.064.235839
AGTCTAG145100.064.0915153
GGCGGTT177650.063.691274
TTAGTTT1138100.062.57152
CCAGACG168300.061.7125636
TGGCCCG567000.060.2973947
AGTTTTA1217600.058.6289334
GGTTATA194350.058.254867
GGAGTCT167100.058.1330641
TTACCCT1278400.055.4515048
GTCGTCT223650.054.831921
TACCCTA1294000.054.755199
CCTCAAC109750.052.0959131
Produced by FastQC (version 0.11.2)