FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16439510
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC185491811.283292506893455TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC362810.2206939257921921TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2113850.067.7064544
GTATGCC2116450.067.6212145
TATGCCG2117000.067.5862846
TCGTATG2120400.067.5738643
ATGCCGT2108550.067.5558347
CTCGTAT2071150.067.4638242
GCCGTCT2087900.067.4203249
TGCCGTC2103900.067.34057648
ACTGACC2102550.067.1736132
CTGACCA2102200.066.9910233
CCGTCTT2101750.066.9616950
CTGCTTG2058050.066.8938157
CAGTCAC2177100.066.887827
GACCAAT2096450.066.8182635
TCTCGTA2088800.066.77244641
CCAATCT2062150.066.7461137
CACTGAC2135450.066.6972731
GTCACTG2159800.066.6962929
ACCAATC2057900.066.59009636
CCAGTCA2186050.066.5845226
Produced by FastQC (version 0.11.2)