FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15728448
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC188140611.961803224323214TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC413290.2627659130767384TruSeq Adapter, Index 3 (98% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG368990.23460038778142636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG274500.17452453032873935No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC237260.15084768694279307TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGC159750.10156755453557782TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2074850.068.5480444
GTATGCC2083400.068.4487945
TATGCCG2064200.068.38949646
TCGTATG2039050.068.3660543
CTCGTAT2054350.068.36188542
GCCGTCT1957150.068.3404449
ATGCCGT1990850.068.1945747
ACTTAGG2254800.068.18989632
CACTTAG2261400.068.18838531
AGGCATC2209550.068.1712436
GGCATCT2100350.068.1533137
TGCCGTC1983500.068.0069748
CCGTCTT1991000.067.8892850
TCTCGTA2074500.067.8577941
GCATCTC2111100.067.78664438
TTAGGCA2257450.067.7820334
CTGCTTG1868950.067.65089457
CATCTCG2110000.067.64619439
ATCTCGT2097150.067.4614940
TAGGCAT2259250.067.4480235
Produced by FastQC (version 0.11.2)