FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17029652
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC13982928.210925273164712TruSeq Adapter, Index 1 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG345820.20306932872145594No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG274500.16118943593210244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1550250.068.15369444
TATGCCG1555300.067.9248446
ATGCCGT1555000.067.8733247
GTATGCC1556950.067.83382445
TCGTATG1562900.067.7242543
TGCCGTC1554500.067.70109648
GCCGTCT1551150.067.639149
CTCGTAT1541300.067.6185342
GATCTCG1529450.067.3370839
CACGATC1516550.067.3178936
CGATCTC1525100.067.2959438
ACGATCT1524500.067.19205537
CCGTCTT1563650.067.10187550
CTGCTTG1542900.066.9915157
TCTCGTA1553000.066.8047241
CATCACG1570500.066.5768133
TCACGAT1554200.066.5116635
ATCTCGT1554750.066.4046140
ATCACGA1569800.066.1864334
TGCTTGA1566100.066.0509958