FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23290698
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT334660.1436882655899793TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC67250.037.58201246
GTATGCC75500.033.4281447
TATGCCG74950.033.11271348
ATGCCGT75450.032.84660349
TGCCGTC78800.031.58292650
GCCGTCT78850.031.11603451
CTCGTAT83600.030.98684344
TCGTATG87200.029.5464845
CCTTTAT103750.024.3940237
CCGTCTT104350.023.91403852
TCTCGTA116400.022.5854943
CAGTCAC130400.022.03821627
TATCTCG117950.021.69511441
CTGCTTG107750.021.07871259
TCCTTTA124450.020.53301836
CCAGTCA143200.020.16574726
ATCTCGT133250.019.7554842
AGTCACA145950.018.92297428
TCCAGTC154850.018.76137525
ACTCCAG158000.018.43152223
Produced by FastQC (version 0.11.2)