FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21522131
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT4258881.978837504520347TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC479750.064.18021446
GCCGTCT480350.063.5147451
ATGCCGT483950.063.4100749
GTATGCC486450.063.25130547
TATGCCG486200.063.20418548
TGCCGTC486200.063.05065550
CTCGTAT493700.062.25574544
TCGTATG495550.062.23410445
CCGTCTT504950.060.63391552
TATCTCG513350.059.81023441
CTGCTTG498350.059.76679659
TCTCGTA522750.058.8693543
ATCTCGT535850.057.5013642
CACACTG542750.057.03027731
CTTCTGC542800.056.14122856
TATATCT547050.056.08812739
GTCACAC560100.055.86413629
CGTCTTC551850.055.5438653
TGCTTGA538900.055.48556560
GATATAT554400.055.45637
Produced by FastQC (version 0.11.2)