FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21604017
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT882220.4083592417095395TruSeq Adapter, Index 7 (97% over 36bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG293300.1357617891154224No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG271250.12555535389552785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC129000.050.47236646
GTATGCC131400.049.52236647
ATGCCGT132650.048.71164349
TATGCCG134100.048.34210248
TGCCGTC136100.047.1929650
GCCGTCT135350.047.03600751
CTCGTAT143700.045.57929244
TCGTATG144650.045.13442645
CGAGTGG147550.043.65188633
ACGAGTG156200.041.34678632
CCGTCTT159500.040.3532152
GGATATC161950.039.7923238
GTGGATA162850.039.4431636
TATCTCG167500.038.55947541
CACGAGT169400.038.37177331
TCTCGTA175450.037.70986643
CTGCTTG160700.037.6311759
AGGGGGG61200.036.8370631
ATCTCGT186500.035.4000942
GTCACGA187450.035.21839529
Produced by FastQC (version 0.11.2)