FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19174317
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG37700.032.86907233
CGTACGT37850.032.83120734
CGTATGC44250.027.84665746
GTACGTA48950.025.7436935
GTATGCC50050.024.68864647
TATGCCG49000.024.5748148
ATGCCGT49950.023.75677549
GCCGTCT49600.023.35683851
TACGTAA55050.023.20900736
TGCCGTC50900.023.03781150
CTCGTAT55450.022.60157644
GTCACCG63000.021.72464429
TCGTATG56900.021.65618545
CACCGTA60550.020.7537631
CGTAATC64150.019.97198738
AGTCACC73450.019.82537528
ACGTAAT67350.019.2303637
TCACCGT77000.018.31960330
ACCGTAC70500.018.02380432
CCGTCTT69850.017.13644252