FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13590622
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT8613216.337612803887857TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGAT1546061.137593261000122TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGGGGGT672610.49490744426561195TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGTAT563800.41484488347921084TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGGT556910.40977521117135035TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGGT492760.362573545199035TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGAT479040.35247834867307765TruSeq Adapter, Index 21 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG454030.3340759532565912No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTTT424980.31270091979601816TruSeq Adapter, Index 21 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG379030.27889084105201367No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTGT274950.20230862134198124TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGTT260410.19161006758925384TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGTGT153530.11296760369025052TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG1712350.068.0621828
GTCACGT1702250.068.0172529
CGGAATC1660900.067.7977138
CGTTTCG1683550.067.7435133
CAGTCAC1730950.067.7072227
GTTTCGG1684700.067.592334
CCAGTCA1733200.067.5895426
TCCAGTC1734050.067.517325
ACTCCAG1737150.067.4633123
CTCGTAT920000.067.3805344
GAACTCC1746850.067.2311321
GGAATCT1322850.067.20788639
CGTATGC699000.067.1823346
TCACGTT1715450.067.1401330
CTCCAGT1745050.067.1255624
ACGTCTG1755100.067.0552915
CGTCTGA1755050.067.0367516
GTATGCC704700.067.0298747
ACGTTTC1710100.066.95499432
TCGTATG806700.066.9027145
Produced by FastQC (version 0.11.2)