FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18842116
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT9764615.182331963140445TruSeq Adapter, Index 20 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210130.11152144483135547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA1087350.068.0939536
CGTATGC1052600.067.4058746
GCCTTAT1100700.067.3544637
GTATGCC1057800.067.1392747
CTCGTAT1076150.067.0092344
TATGCCG1044250.066.9845448
TCGTATG1072400.066.8373745
ATGCCGT1046200.066.7087649
CGTGGCC1114350.066.68050433
TGCCGTC1049050.066.45254550
GCCGTCT1022650.066.4482851
AGTCACG1148600.066.3948428
GTCACGT1141750.066.35406529
TGGCCTT1117250.066.2211235
GTGGCCT1118600.066.1775234
CCTTATC1119550.066.0901338
TATCTCG1099250.065.8744341
TCTCGTA1100400.065.7698943
CTTATCT1102900.065.7237739
CCGTCTT1040200.065.6619252
Produced by FastQC (version 0.11.2)