FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13134824
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT174716213.30175417653103TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTAT229930.17505373501769037TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTAT141380.10763752906015338TruSeq Adapter, Index 19 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1948750.068.36085546
GTATGCC1952550.068.1926947
TATGCCG1948500.068.17222648
GCCGTCT1936350.068.16405551
CTCGTAT1937350.068.1537144
TCGTATG1952050.068.11030645
ATGCCGT1949550.068.079649
TGCCGTC1950300.068.0095750
ACGATCT1879200.067.96924639
GTCACGT1964750.067.9178729
AGTCACG1975500.067.9117728
CGATCTC1884300.067.9089340
CCGTCTT1948100.067.88328652
GATCTCG1914850.067.81402641
AACGATC1886150.067.8137638
TCTCGTA1950800.067.6784443
CACGTGA1962350.067.63602431
TCACGTG1965600.067.6106830
CTGCTTG1902300.067.5336759
CAGTCAC2002850.067.5253527
Produced by FastQC (version 0.11.2)