FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22508594
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT241070110.71013587077007TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2705000.068.0800946
GTATGCC2711600.067.9158547
ACGTCCG2692700.067.8709432
TCGTATG2715550.067.8258345
GTCCGCA2672600.067.8174934
CTCGTAT2711800.067.812744
CGTCCGC2677550.067.8111833
CGCACAT2661750.067.7704237
TATGCCG2701250.067.7669848
TCCGCAC2668000.067.708635
GCCGTCT2657550.067.7040351
ATGCCGT2702350.067.6488949
CACGTCC2716800.067.56895431
AGTCACG2775950.067.5071728
GTCACGT2765100.067.487929
TGCCGTC2703500.067.4799850
CCGCACA2661450.067.46458436
TCACGTC2754100.067.3275130
GCACATC2690950.067.2849838
TCTCGTA2735800.067.2668843