FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11208150
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT9634438.595914580015435TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT143130.12770171705410793TruSeq Adapter, Index 16 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA1043300.068.1239336
CGTCCCG1056800.068.09894635
CCGTCCC1059900.068.0420834
CCCGTCC1066000.067.91482533
CACCCGT1071050.067.8768331
ACCCGTC1069050.067.8657432
CGTATGC1077600.067.8470346
GTATGCC1079400.067.7087747
GTCACCC1083250.067.63727629
TCACCCG1078550.067.6286930
CCCGATC1043550.067.6257938
TCGTATG1080650.067.5304145
CTCGTAT1072200.067.5144344
TATGCCG1079000.067.47932448
ATGCCGT1079100.067.43120649
GCCGTCT1065900.067.42284451
TGCCGTC1079150.067.287750
AGTCACC1101750.067.2230328
CCGATCT1050800.067.0298839
TCCCGAT1061250.067.0076737
Produced by FastQC (version 0.11.2)