FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24605159
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT4068891.6536735243206517TruSeq Adapter, Index 14 (97% over 44bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG479260.1947802897758149No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400490.16276667832140407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC455300.063.94378746
GTATGCC461950.063.29363347
ATGCCGT454750.062.9016549
TATGCCG456550.062.6921148
CCGTATC521050.062.37006838
CGTATCT498900.062.34908339
TGCCGTC458050.062.2183350
TCGTATG481300.062.02538345
CTCGTAT488450.061.97091344
GCCGTCT444400.061.9584951
GTTCCGT535750.060.6517235
TCCGTAT541500.060.36309437
GTATCTC517250.059.72532740
AGTTCCG550500.059.1420434
GTCACAG568350.058.68608529
TATCTCG528450.058.51805541
CCGTCTT477250.058.35912752
CAGTCAC585800.058.26312327
TCTCGTA527500.057.83967243
CCAGTCA591150.057.6824326
Produced by FastQC (version 0.11.2)