FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27893563
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT3024261.0842143042106167TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC367300.061.02313246
GTATGCC370900.060.38179847
TATGCCG373100.059.63160748
ATGCCGT372950.059.5423249
TGCCGTC378800.058.43755350
TCGTATG384700.058.3732945
CTCGTAT382700.058.3040544
GCCGTCT376550.058.14104551
CCGTCTT408750.053.76446552
TCTCGTA426700.052.5127643
CTGCTTG397800.052.32682859
AGTCAAC445750.050.21987534
CACAGTC452450.050.1550631
ATCTCGT446600.050.0463642
GTCAACA451500.049.53334835
CAATCTC450800.049.36506340
AATCTCG460400.048.29667341
ACAGTCA469750.048.20517732
GTCACAG478350.047.90759329
TGCTTGA442600.047.29232860
Produced by FastQC (version 0.11.2)