FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19323581
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2644951.3687680352829013TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC323300.063.5151344
GTATGCC328900.062.5712545
TATGCCG327750.062.49028446
ATGCCGT320450.062.45987747
GCCGTCT320300.061.570549
TGCCGTC323850.061.45777548
TCGTATG336700.061.0283143
CTCGTAT335600.060.43498242
CCGTCTT344700.057.62745750
CCTTGTA365500.057.17385533
GTAATCT359400.056.55672537
CTGCTTG335250.056.43610857
TCTCGTA363350.055.9258141
TGTAATC367100.055.6177736
ACCTTGT381100.054.88973232
TAATCTC371650.054.76003638
CACCTTG384300.054.7494931
ATCTCGT374800.054.31139840
GTCACCT391900.054.1160529
AGTCACC402600.053.29521628
Produced by FastQC (version 0.11.2)