FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13151464
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC189435314.404122613269518TruSeq Adapter, Index 11 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG498310.37890078245281283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG492010.37411044124061016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2025900.068.97652444
GTATGCC2027500.068.9008545
TATGCCG2029900.068.7959146
TCGTATG2034050.068.7406643
ATGCCGT2022450.068.7182247
GCCGTCT1999900.068.7075349
CTCGTAT2025500.068.64464642
TGCCGTC2016550.068.52862548
CCGTCTT2010650.068.3991150
TCTCGTA2029950.068.27975541
CTGCTTG1967750.068.1806457
CATCTCG2026250.068.0692739
GGCTACA2025700.068.0687534
GCTACAT2022650.068.0530935
CGGCTAC2035000.067.96889533
ACGGCTA2045950.067.96294432
ATCTCGT2038950.067.9364840
CTACATC2019550.067.9282436
TGCTTGA1984300.067.7497858
TACATCT2027200.067.7441737
Produced by FastQC (version 0.11.2)