FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16652358
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC188779611.336508619379911TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC1366650.8206945827131509TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC651110.39100168276468716TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATGC432710.25984908563700104TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATGC314210.18868799241524833TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTGC180860.10860924320747849TruSeq Adapter, Index 10 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2149450.068.4254944
GTATGCC2179550.068.3004245
CACTAGC2584500.068.2541631
CTCGTAT2092050.068.25236542
TATGCCG2130050.068.24341646
TCGTATG2028700.068.21017543
ACTAGCT2572600.068.16897632
GCCGTCT1924150.068.0786149
ATGCCGT1962450.068.05681647
TAGCTTA2565750.068.0281834
CTAGCTT2566600.068.0081833
TCACTAG2605000.067.9010430
GCTTATC2536600.067.85103636
TGCCGTC1957100.067.83465648
TATCTCG2217500.067.6964339
TCTCGTA2126500.067.68799641
CTTATCT2254450.067.5916137
ACGTCTG2651600.067.59054615
CAGTCAC2638900.067.587927
CCAGTCA2637450.067.5850426
Produced by FastQC (version 0.11.2)