FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19274405
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC419943721.787634949042527TruSeq Adapter, Index 9 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG733830.38072770599144307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG595220.30881368322394387No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC442900.229786600416459TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4517450.069.1223744
GTATGCC4521700.069.0541245
TATGCCG4523400.069.0425846
CTCGTAT4463250.069.0369442
TCGTATG4531700.069.0041343
ATGCCGT4521200.069.00004647
GCCGTCT4502350.068.9797449
TGCCGTC4517800.068.9239648
GATCTCG4431200.068.8788639
CACGATC4516050.068.77328531
CAGATCT4430400.068.766537
AGTCACG4553500.068.7649328
CCGTCTT4517750.068.7617150
AGATCTC4441150.068.7097238
GTCACGA4545150.068.7074929
TCTCGTA4469100.068.7069341
CTGCTTG4482400.068.69919657
CGATCAG4508500.068.6864733
ATCTCGT4464200.068.5308140
ACGATCA4531500.068.5298932
Produced by FastQC (version 0.11.2)