FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26032717
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC23198988.911470900252171TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC1509520.5798549571295228TruSeq Adapter, Index 8 (98% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1470450.5648469193592048No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG917090.35228362832815335No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC848470.32592448955673736TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC753410.2894089003464371TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGC511930.19664870170869989TruSeq Adapter, Index 8 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2654850.068.3977544
GTATGCC2683000.068.2773145
TATGCCG2612950.068.2427446
CTCGTAT2591550.068.21641542
TCGTATG2529350.068.0903243
GCCGTCT2340450.067.85982549
ATGCCGT2390950.067.8509947
TGCCGTC2376950.067.6386948
TCTCGTA2641550.067.48225441
GTCACAC3337200.067.28918529
CCGTCTT2443900.067.2767950
CTGCTTG2333750.067.0672657
AATCTCG2812600.067.0511439
ATCTCGT2702000.066.9954940
CAGTCAC3389650.066.8768627
ACACTTG3325950.066.8276432
GAATCTC2840250.066.7030338
CCAGTCA3397150.066.69688426
CACTTGA3328050.066.6575633
AGTCACA3390700.066.5961228
Produced by FastQC (version 0.11.2)