FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12931999
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC204084715.781373011241339TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC819980.6340705717654325TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGC364060.28151873503856595TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC231570.1790674434787692TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGATGC196690.15209558862477487TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGC192140.1485771843935342TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGG149520.11562017596815466TruSeq Adapter, Index 7 (97% over 49bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC136640.10566038552895032TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2300650.068.6389744
GTATGCC2314750.068.5013645
CTCGTAT2250800.068.49599542
TATGCCG2286150.068.4627646
TCGTATG2233850.068.4278443
GCCGTCT2077400.068.40787549
ATGCCGT2121200.068.34383447
TGCCGTC2109450.068.26678548
CATCTCG2350500.068.1237239
TCTCGTA2272650.068.1157641
CCGTCTT2110350.068.08462550
CTGCTTG1993050.068.0532457
ATCTCGT2313700.067.8947640
CTTCTGC2247300.067.7679154
AGTCACC2655650.067.7638928
ACCAGAT2628700.067.6394232
CAGTCAC2681350.067.5948127
CCAGATC2609600.067.59099633
GATCATC2579550.067.5598436
AGATCAT2605950.067.5416535
Produced by FastQC (version 0.11.2)