FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33835557
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2035740.6016570083359348TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC261750.057.5351544
GTATGCC268950.055.9424145
TATGCCG269250.055.93092746
ATGCCGT270850.055.39193347
GCCGTCT269550.054.84029849
TGCCGTC274950.054.08167648
TCGTATG285350.052.89826643
CTCGTAT287800.052.27712242
CCGTCTT309500.047.89593550
CTGCTTG318700.045.50712257
TATCTCG328650.045.2257339
TCTCGTA334850.045.07811441
ATCTCGT357700.042.2969440
CACGCCA355250.041.7476631
GCCAATA359150.041.2272634
CGCCAAT361050.041.0292133
TCACGCC368050.040.38022630
CTTCTGC370050.039.99757854
ACGCCAA376000.039.5287232
GTCACGC383200.039.12318829
Produced by FastQC (version 0.11.2)