FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18173659
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC8917644.906903997703489TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1889781.039845635928351TruSeq Adapter, Index 5 (98% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG315950.17385051628843703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG272800.15010736142897807No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC189770.10442035915827408TruSeq Adapter, Index 5 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1239200.067.80675544
GTATGCC1244700.067.64194545
TATGCCG1234950.067.4631946
GCCGTCT1163700.067.29536449
TCGTATG1211950.067.18861443
ATGCCGT1187400.067.11478447
TGCCGTC1182450.066.9655748
CTCGTAT1017500.066.7860242
CCGTCTT1193000.066.4186850
GATCTCG1041300.065.810439
CTGCTTG1135200.065.7691257
TCTCGTA1040400.065.46603441
AGTGATC1079400.065.3800136
CACAGTG1373600.065.03419533
CTTCTGC1255250.064.86822554
ATCTCGT1053850.064.6520440
TGCTTGA1158000.064.6379258
GTGATCT1062000.064.5850437
ACACAGT1392550.064.4043232
CAGTGAT1375700.064.3772635
Produced by FastQC (version 0.11.2)