FastQCFastQC Report
Thu 15 Dec 2016
H7TNYBGX2_n01_las01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TNYBGX2_n01_las01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17082672
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC182246410.668494952077754TruSeq Adapter, Index 2 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2980601.7448090088014334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2120311.2412051229456376No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC588070.3442494242118563TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC250330.14654030704330095TruSeq Adapter, Index 2 (98% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245480.14370117274393607No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC198570.11624059748966671TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2010000.068.638444
TATGCCG1999900.068.4757546
GTATGCC2023400.068.4594645
TCGTATG1962300.068.4243743
GCCGTCT1873300.068.36587549
CTCGTAT1981900.068.36133642
ATGCCGT1906150.068.3121447
TGCCGTC1897700.068.2806148
CCGTCTT1913600.067.78548450
GTATCTC2052350.067.7776438
TCTCGTA2010250.067.7327841
CTGCTTG1798700.067.698757
TATCTCG2051900.067.62459639
ATCTCGT2033850.067.3767940
CGATGTA2213900.067.2293534
TGTATCT2079850.067.15542637
CTTCTGC1995250.067.12874654
ATGTATC2206700.067.0529136
CCGATGT2222950.066.9792733
GATGTAT2226750.066.91370435
Produced by FastQC (version 0.11.2)