FastQCFastQC Report
Mon 28 Jun 2021
H7TLNDRXY_l01_n02_Fluc_bc_C08_T0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TLNDRXY_l01_n02_Fluc_bc_C08_T0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGC431.4357262103505843No Hit
GGCAAGACGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC250.8347245409015025No Hit
TTGGTAAAGCTTAAGGCCCGAATTCACATTAGATCGGAAGAGCGTCGTGT220.7345575959933223No Hit
TGCGGCCTGCTTTAGGCTCGAATTCAACCGAAATTGTAGTAGATCGGAAG210.7011686143572621No Hit
CCATCTCTGCTTAAGGCCGAATTCGTCCCAGATCGGAAGAGCGTCGTGTA190.6343906510851419Illumina Single End PCR Primer 1 (100% over 21bp)
CAAATCACGCTTAAGCCGAATCAAGCTTAGATCGGAAGAGCGTCGTGTAG150.5008347245409015Illumina Single End PCR Primer 1 (100% over 22bp)
ACTTGTATGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC150.5008347245409015No Hit
TGGATGCAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC140.46744574290484137No Hit
CGGCATTTGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC140.46744574290484137No Hit
GTCGCGACGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC140.46744574290484137No Hit
CTCGTATTGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC140.46744574290484137No Hit
CTGGACATGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC130.4340567612687813No Hit
CTTTTACTGCTTAAGGCCGAATTCAGCTTAGATCGGAAGAGCGTCGTGTA130.4340567612687813Illumina Single End PCR Primer 1 (100% over 21bp)
GGAGGGAAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC130.4340567612687813No Hit
TGCACCCTGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC120.40066777963272115No Hit
CTCGAAGAGCTTAAGGCCCGAATTCATTTAAGATCGGAAGAGCGTCGTGT120.40066777963272115No Hit
ACCACAGCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC110.36727879799666113No Hit
TATTCACCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC110.36727879799666113No Hit
CTTCTTGTGCTTAAGGCCCGAATCAGGTATAGATCGGAAGAGCGTCGTGT110.36727879799666113No Hit
AATGTTAAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC110.36727879799666113No Hit
ATAAGGGGGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC110.36727879799666113No Hit
TGTCAGCAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC110.36727879799666113No Hit
TCAACGATGCTTAAGGCTCGAATTCAGCTTAGATCTGATATCGGTACCCA110.36727879799666113No Hit
GTAGACAAGCTTAAGGCCGAATTCAAGCTAGATCGGAAGAGCGTCGTGTA100.333889816360601Illumina Single End PCR Primer 1 (100% over 21bp)
AGAGTACTGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC100.333889816360601No Hit
TTGGTGTAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC90.3005008347245409No Hit
CCGGCAATGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC90.3005008347245409No Hit
ACATTCTAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC90.3005008347245409No Hit
CGCACAATGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC90.3005008347245409No Hit
TCCAGCACGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC90.3005008347245409No Hit
CACACCAGGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC80.2671118530884808No Hit
AAACGCCTGCTTAAGGCTCGAATTCAACCGAAGCCCATGAAGATCGGAAG80.2671118530884808No Hit
GGGGACACGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC80.2671118530884808No Hit
TCATTCCTGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC80.2671118530884808No Hit
CATTCACCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC80.2671118530884808No Hit
GCACTTTCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC80.2671118530884808No Hit
CTCGCCTAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC70.23372287145242068No Hit
CCAGCACAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC70.23372287145242068No Hit
AGACGTGCGCTTAAGGCCGAATTCAGCTTAGATCGGAAGAGCGTCGTGTA70.23372287145242068Illumina Single End PCR Primer 1 (100% over 21bp)
CACTTCCAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC70.23372287145242068No Hit
ATGCTTTCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC70.23372287145242068No Hit
ATGTCCTCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC70.23372287145242068No Hit
ATGGAGCTGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC70.23372287145242068No Hit
TGAAGACTGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC70.23372287145242068No Hit
AATTTAGCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC70.23372287145242068No Hit
GTATTATTGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC70.23372287145242068No Hit
ACTACCCCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC60.20033388981636058No Hit
GGTCCCCGGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC60.20033388981636058No Hit
CGACTTCCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC60.20033388981636058No Hit
ACTGGCAAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC60.20033388981636058No Hit
CCTATATCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC60.20033388981636058No Hit
TGTCCACAGCTTAAGGCCGAATTCAGCTTAGATCGGAAGAGCGTCGTGTA60.20033388981636058Illumina Single End PCR Primer 1 (100% over 21bp)
GGACTCTCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC50.1669449081803005No Hit
TATTTAGCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC50.1669449081803005No Hit
ACATTAGCGCTTAAGGCCGAATCAAGCTTAGATCGGAAGAGCGTCGTGTA50.1669449081803005Illumina Single End PCR Primer 1 (100% over 21bp)
GGAAGGTAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC50.1669449081803005No Hit
CCAGGAACGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC50.1669449081803005No Hit
TATTGTCAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC40.1335559265442404No Hit
AATGCCTGGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC40.1335559265442404No Hit
TCATCTTCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC40.1335559265442404No Hit
CCCTATGCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC40.1335559265442404No Hit
TCTGTTTGGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC40.1335559265442404No Hit
GATTATCTGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC40.1335559265442404No Hit
CCTGAGTTGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC40.1335559265442404No Hit
GTGCACTGGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
TTCGGCCAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
GGGCGAAAGCTTAAGGCTCGAATTCAATCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
AGTACTGCGCTTAAGGCCGAATCAAGCTTAGATCGGAAGAGCGTCGTGTA30.10016694490818029Illumina Single End PCR Primer 1 (100% over 21bp)
ACGCAATCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
CGCTCGACGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
TCTAAGCAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
CTTCGCGTGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
GGGTTTACGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
TGACGAATGCTTAAGGCTCGATAGTTCATATTAAACCGAACATGTGAGAG30.10016694490818029No Hit
AGGTCAGAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
CCGATGGGGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
GTTACGCAGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
CTCAACCCGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
ACTGCAAGGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit
CTTGTGATGCTTAAGGCTCGAATTCAAGCTTAGATCTGATATCGGTACCC30.10016694490818029No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)