FastQCFastQC Report
Mon 28 Jun 2021
H7TLNDRXY_l01_n01_Fluc_bc_C08_T0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TLNDRXY_l01_n01_Fluc_bc_C08_T0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTTGATTTGGTCATTGGTAAAATACTGACCAGCCGTTTGAGCTTGAG451.5025041736227045No Hit
AATGTGAATTCGGGCCTTAAGCTTTACCAAAGATCGGAAGAGCGGTTCAG230.7679465776293823No Hit
GGGACGAATTCGGCCTTAAGCAGAGATGGAGATCGGAAGAGCGGTTCAGC220.7345575959933223Illumina Paired End PCR Primer 2 (100% over 21bp)
ACTACAATTTCGGTTGAATTCGAGCCTAAAGCAGGCCGCAAGATCGGAAG190.6343906510851419No Hit
GCAGGCAATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC150.5008347245409015No Hit
CGTTTCCCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC150.5008347245409015No Hit
ACCCCAGATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC140.46744574290484137No Hit
AAGCTTGATTCGGCTTAAGCGTGATTTGAGATCGGAAGAGCGGTTCAGCA130.4340567612687813Illumina Paired End PCR Primer 2 (100% over 22bp)
CCAACGAATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC130.4340567612687813No Hit
CGAAATATTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC130.4340567612687813No Hit
CGAGCCATTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC130.4340567612687813No Hit
TCACTACTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC130.4340567612687813No Hit
CCTTGTTTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC130.4340567612687813No Hit
CACCATTTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC120.40066777963272115No Hit
AGCTTGAATTCGGCCTTAAGCTTGTCTACAGATCGGAAGAGCGGTTCAGC120.40066777963272115Illumina Paired End PCR Primer 2 (100% over 21bp)
TAAATGAATTCGGGCCTTAAGCTCTTCGAGAGATCGGAAGAGCGGTTCAG120.40066777963272115No Hit
AAGCTGAATTCGGCCTTAAGCAGTAAAAGAGATCGGAAGAGCGGTTCAGC120.40066777963272115Illumina Paired End PCR Primer 2 (100% over 21bp)
GGTAAACTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC120.40066777963272115No Hit
GGATATACTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC120.40066777963272115No Hit
CAATTTATTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC110.36727879799666113No Hit
CGCTCCCTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC110.36727879799666113No Hit
ATACCTGATTCGGGCCTTAAGCACAAGAAGAGATCGGAAGAGCGGTTCAG110.36727879799666113No Hit
CTGAACACTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC110.36727879799666113No Hit
CCTCCATCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC100.333889816360601No Hit
CCATTTGGTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC100.333889816360601No Hit
CCCTCACTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC100.333889816360601No Hit
CACCTGCTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC100.333889816360601No Hit
TTGTCGTATTCGGTTTAAATGGACTAAAGGAGGCTTTTGTCGACGGATCC90.3005008347245409No Hit
ACTGTCTCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC90.3005008347245409No Hit
TGCTTCAATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC90.3005008347245409No Hit
TATCGAAGTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC90.3005008347245409No Hit
CAGCAATATTCGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATCC80.2671118530884808No Hit
TGGGTGAATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC80.2671118530884808No Hit
TCATGGGCTTCGGTTGAATTCGAGCCTTAAGCAGGCGTTTAGATCGGAAG80.2671118530884808No Hit
GTTATCGCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC80.2671118530884808No Hit
TCATTCTCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC80.2671118530884808No Hit
CCTTGACCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC70.23372287145242068No Hit
CCGTATCATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC70.23372287145242068No Hit
GATTGTTTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC70.23372287145242068No Hit
GAGACCCCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC70.23372287145242068No Hit
TGCATTAGTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC70.23372287145242068No Hit
TTTCACCATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC70.23372287145242068No Hit
GCCAAAGCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC70.23372287145242068No Hit
GTGTCCCTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC70.23372287145242068No Hit
CAATTGGTTTCGGTTTAATATGGCTAAAGGAGGCTTTTGTCGACGGATCC70.23372287145242068No Hit
CGGCATCTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC60.20033388981636058No Hit
GTTCATCCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC60.20033388981636058No Hit
GCCGTATTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC60.20033388981636058No Hit
AAGCTGAATTCGGCCTTAAGCTGTGGACAAGATCGGAAGAGCGGTTCAGC60.20033388981636058Illumina Paired End PCR Primer 2 (100% over 21bp)
TTTGTAATTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC60.20033388981636058No Hit
GGCACCGTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC60.20033388981636058No Hit
ATCCATGCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC60.20033388981636058No Hit
CGAAAGTTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC60.20033388981636058No Hit
AAGCTGAATTCGGCCTTAAGCGCACGTCTAGATCGGAAGAGCGGTTCAGC60.20033388981636058Illumina Paired End PCR Primer 2 (100% over 21bp)
AAGCTTGATTCGGCCTTAAGCGCTAATGTAGATCGGAAGAGCGGTTCAGC50.1669449081803005Illumina Paired End PCR Primer 2 (100% over 21bp)
TTGACTCTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC50.1669449081803005No Hit
CAGTTGCCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC50.1669449081803005No Hit
TCTTCCTATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC50.1669449081803005No Hit
TCCGGCGCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC40.1335559265442404No Hit
GCCGAACGTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC40.1335559265442404No Hit
GACAACGGTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC40.1335559265442404No Hit
TCCGATTGTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC40.1335559265442404No Hit
CCACTGTATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC40.1335559265442404No Hit
GGCAAAATTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
ATATCTTATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
AATCCGTATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
AAGCTTGATTCGGCCTTAAGCGCAGTACTAGATCGGAAGAGCGGTTCAGC30.10016694490818029Illumina Paired End PCR Primer 2 (100% over 21bp)
GAGCACATTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
GGGCCGCATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
GGTTCCCTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
ATCACACTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
CTGCAAATTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
CAACCGTGTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
CTCACATGTTCGGTTTAATATGAACTATCGAGCCTTAAGCATTCGTCAAG30.10016694490818029No Hit
CGTAGATATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
CATCGCCATTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
CCACAAGCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
CCCCTTCCTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
CTTCCGATTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
CCAATCCGTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
CTCTGCACTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit
TTGCCGTTTTCGGTTTAATATGGACTAAAGGAGGCTTTTGTCGACGGATC30.10016694490818029No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)