FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30959907
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1649430.5327632282616352No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT740960.2393288842889612No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG322200.10407008005547305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT208950.038.7889183
TACTCTA213850.038.6631434
GCACACG49700.038.22667311
TTGTACT201800.038.225021
ACTCTAG247650.037.525655
TGTACTC222150.036.3898242
GTGGGCG43950.036.3820271
CTCTAGT228950.035.730716
TCTAGTT232250.035.1772547
ACGTCTG52800.034.8566915
CTAGTTG240350.033.627878
CACACGT61500.030.721612
ACACGTC60450.030.6183513
GTTACCT263350.030.58479514
CACGTCT60200.030.223214
ACCTCTA276450.029.94570517
ACCTACT223800.029.74633433
TGTTACC273950.029.4389313
CTAATGC273800.029.3961521
GGACACC261000.029.35755229
Produced by FastQC (version 0.11.5)