FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-9R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-9R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30087195
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT809980.269210871934057No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT501660.16673538360754467No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG318640.10590551894252688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG328700.035.53755
TACTCTA225650.034.986784
TGTACTC239650.032.8988272
TTGTACT240300.032.47571
GTACTCT249600.031.6576653
CTCTAGT262600.030.5570246
TCTAGTT263600.030.3080377
CTAGTTG267300.029.3516988
GCTGACT132250.028.3136311
ACCTCTA303200.025.6687317
TAGTTGT311600.025.6055039
GGACACC294200.025.58426329
GTTACCT309050.025.1714814
ACCTACT248550.025.04217333
CTAATGC310050.024.9475521
ACACCTA254000.024.65760231
GACACCT309600.024.33419430
TTACCTC325050.024.06173315
TACCTCT324900.023.9436716
ATGCTGG319500.023.86161624
Produced by FastQC (version 0.11.5)