FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-8R-FBH3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-8R-FBH3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28012074
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1064390.3799754348785456No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA323300.11541451732563607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA272300.036.7425774
TTGTACT271250.036.5629921
TGTACTC280850.035.760822
GTACTCT282000.035.627523
ACTCTAG308550.034.9323845
TCTAGTT313350.032.1742867
CTCTAGT317800.031.5924136
CTAGTTG325350.030.4499598
ACCTACT288550.028.0858833
GGACACC350850.027.47027829
ACCTCTA362100.027.38839717
CTAATGC365150.026.96179421
ACACCTA301750.026.93944231
GTTACCT367950.026.80102314
TAGTTGT380800.026.3928289
GACACCT365050.026.39189130
TACCTCT380150.026.00520916
TTACCTC381300.025.8992915
ATGCTGG378950.025.74197624
TGTTACC389000.025.5394713
Produced by FastQC (version 0.11.5)