FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-8G-GBF2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-8G-GBF2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27400168
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1351900.49339113541201646No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA406740.14844434530474412No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308910.11274018465872179No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA286030.10438987089422226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT323750.039.939581
TACTCTA331650.039.5259784
GTACTCT339550.038.709283
ACTCTAG353400.038.558875
TGTACTC340550.038.3898242
CTCTAGT360450.036.3970386
TCTAGTT370000.035.5328757
CTAGTTG378950.034.2689448
GGACACC406650.030.87247529
ACCTCTA421950.030.59471917
ACCTACT336150.030.56528333
GTTACCT426400.030.22557814
CTAATGC427200.029.92066621
ACACCTA348200.029.69016831
GACACCT422650.029.67051530
TACCTCT442750.029.14133316
TAGTTGT451750.029.1104899
ATGCTGG436400.028.90692724
TTACCTC449250.028.8441215
TGTTACC449350.028.79855313
Produced by FastQC (version 0.11.5)