FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-7R-GBF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-7R-GBF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22663534
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1981310.8742281764176761No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA866160.3821822315972434No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG674520.2976234862577037No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC473280.2088288613770474No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA349630.15426985041256142No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG339920.1499854347517029No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT314100.1385926837359081No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281570.12423922941585369No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA251320.11089179648681446No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG235420.10387612099684013No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT228430.10079187120596461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG170200.056.37755211
ACGTCTG174700.054.7852615
ACACGTC179600.053.38786713
CACACGT180450.053.25274312
AGCACAC186400.051.66586710
CACGTCT186050.051.5748614
ATGGGGG98900.050.6496940
TTGTACT370650.049.1627661
CATGGGG113400.047.87694539
GCATGGG116100.047.8471638
CGTCTGA201750.047.5093116
TCCAGTC202150.047.15101625
GAGCACA205550.046.9543769
CAGTCAC198600.046.40858527
AGTCACC201500.045.46462628
CCAGTCA205950.045.21155526
GGCATGG135400.044.49242837
AGAGCAC222050.043.4652188
CTGAACT221850.043.3965719
ACTCCAG222650.043.1837723
Produced by FastQC (version 0.11.5)