FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-7G-HB7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-7G-HB7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25451684
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT615470.2418189696210278No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT369770.14528311761217844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA167800.034.1396564
ACTCTAG252450.033.8510935
GTACTCT179550.031.8471013
GCACACG50450.030.31232811
CTAGTTG192350.029.3820388
TATGCCG46900.028.96154646
CTCTAGT203450.028.6049566
TCTAGTT203200.028.4847587
GTATGCC49250.028.00644545
CGTATGC50750.027.3164744
GGACACC201100.026.861429
ACCTACT171650.026.12479233
GACACCT210200.025.79839530
ACCTCTA217700.025.78368417
ACGTCTG60350.025.51365715
CTAATGC219100.025.3354721
GCTGACT113900.025.1943821
GTTACCT222100.025.06800714
ACACCTA180350.024.94350831
ATGCTGG224000.024.59621824
Produced by FastQC (version 0.11.5)