FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-6R-DREB2A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-6R-DREB2A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27114107
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT981690.3620587615148085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA248800.038.1736564
GTACTCT254300.037.2101483
TTGTACT251550.037.1723861
ACTCTAG314800.036.573415
TGTACTC258600.036.5371552
CTCTAGT283550.033.7793926
TCTAGTT286450.033.510347
CTAGTTG284300.033.0745168
ACCTACT260600.029.605833
GGACACC309050.029.23622729
CTAATGC320550.029.02206821
ACCTCTA324900.028.89860217
ACACCTA267200.028.8366531
GTTACCT324900.028.71544314
TAGTTGT331600.028.4833459
GACACCT325400.027.81010830
TACCTCT339100.027.59562916
ATGCTGG333450.027.5449224
TTACCTC344400.027.24198515
TGTTACC346000.027.10569613
Produced by FastQC (version 0.11.5)