FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-6G-ABF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-6G-ABF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28423262
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT666480.23448399413128584No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT373640.1314557069487661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG274200.034.4084365
TACTCTA196100.033.1752554
GTACTCT211000.030.8490813
CTAGTTG213050.030.3873448
TCTAGTT221100.029.8191647
CTCTAGT222650.029.5022816
GGACACC226700.027.37931329
ACCTACT186500.026.89736633
ACCTCTA240300.026.59200717
GACACCT235400.026.33771930
CTAATGC242900.025.99461621
ACACCTA195750.025.87780831
GCACACG52450.025.48696511
GTTACCT251250.025.43297814
ATGCTGG253350.024.84117724
TACCTCT261450.024.42745816
TTACCTC265100.024.15705115
CCTCTAA266400.023.9216618
TGGACAC259700.023.91240728
GCTGACT126500.023.5156881
Produced by FastQC (version 0.11.5)