FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-5R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-5R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29022422
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1253230.43181440887324984No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT487730.16805282481248462No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA354920.12229165436296117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT286000.041.32111
TACTCTA291250.040.8758624
TGTACTC303500.039.3411832
ACTCTAG411150.038.2159465
GTACTCT313350.038.048873
TCTAGTT326850.036.552027
CTCTAGT332450.036.073656
CTAGTTG341250.034.5789648
TAGTTGT387150.030.8407259
ACCTACT315900.030.35291533
GTTACCT388550.030.2791314
GGACACC379050.030.21137229
ACCTCTA391800.030.06394817
CTAATGC391450.029.8271121
ACACCTA323800.029.67887531
GACACCT391550.029.3092830
ATGCTGG394850.029.28033624
TTACCTC404650.029.14353415
TGTTACC405800.029.1296813
TACCTCT406900.028.9309316
Produced by FastQC (version 0.11.5)