FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-4R-FBH3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-4R-FBH3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21012177
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT989010.4706842132540574No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA283250.13480278602260012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT227000.040.8699951
TACTCTA235650.039.992094
ACTCTAG260800.038.9533355
TGTACTC242050.038.8335572
GTACTCT243950.038.4452133
CTCTAGT270500.034.956296
TCTAGTT270100.034.85257
CTAGTTG280500.033.2858128
ACCTACT244350.030.29979533
GGACACC302200.029.86447529
ACCTCTA310900.029.80591417
GTTACCT310750.029.76406714
TAGTTGT315800.029.720289
CTAATGC311700.029.49715821
ACACCTA256750.029.0151331
TACCTCT321600.028.85776516
GACACCT313700.028.76925730
TTACCTC326800.028.35575115
TGTTACC329150.028.19576313
ATGCTGG323750.028.10999524
Produced by FastQC (version 0.11.5)