FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-4G-GBF2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-4G-GBF2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24006435
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1363010.5677686003773571No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA390650.16272720210226965No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA278230.11589809149088567No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC270050.11249067177196448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT304150.041.3178671
TACTCTA312050.040.899524
GTACTCT325550.039.0849233
ACTCTAG338700.039.0452655
TGTACTC332300.038.396062
CTCTAGT350550.036.3980336
TCTAGTT352700.036.1655547
CTAGTTG365500.034.382218
TATGCCG53000.030.91262446
GTTACCT406050.030.59516514
ACCTCTA409300.030.54881317
GCACACG56900.030.38171811
CGTATGC54700.030.33596644
ACCTACT326600.030.26862533
CTAATGC409350.030.25883121
GGACACC399550.030.24652729
TAGTTGT424700.029.9603239
GACACCT409350.029.70170230
ACACCTA334450.029.63323831
TACCTCT425050.029.4086716
Produced by FastQC (version 0.11.5)