FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-3R-GBF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-3R-GBF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23140824
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1374640.5940324337629463No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA366020.15817068571110518No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC282780.12219962435218383No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA271830.11746772716477165No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG239930.10368256549550699No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT232190.10033782720961015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT297800.043.704271
TACTCTA316100.041.438024
TGTACTC317500.041.15612
GTACTCT320950.040.735423
ACTCTAG376000.039.443625
CTCTAGT351000.037.3778086
TCTAGTT356000.036.6458557
CTAGTTG379450.034.0860258
TAGTTGT413750.031.4295089
GTTACCT419350.030.6928914
ACCTACT335100.030.4512333
ACCTCTA422700.030.34214817
CTAATGC421550.030.12258121
GGACACC415200.030.093229
TGTTACC429100.030.06053513
ACACCTA342050.029.9774631
TACCTCT431600.029.72450616
TTACCTC435350.029.63720915
ATGCTGG433300.029.22836924
GACACCT428900.029.13936430
Produced by FastQC (version 0.11.5)