FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-3G-HB7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-3G-HB7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26041206
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1033350.39681341947066506No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT317670.12198743790898163No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA303400.11650766097391957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA253600.038.7344674
ACTCTAG331750.037.331385
GCACACG61800.036.9765711
GTACTCT266900.036.8174553
GTATGCC56900.034.63902745
CGTATGC57300.034.21359644
CTCTAGT291000.034.033016
TATGCCG57950.033.7691746
TCTAGTT292650.033.7091377
ACGTCTG68100.033.19637315
CTAGTTG300600.032.375318
ACACGTC75150.029.98889413
AGTCACG75050.029.94502428
CACACGT78300.029.09529712
GGACACC325000.028.93213729
ACCTCTA336100.028.78929317
ACCTACT270250.028.53568533
GTTACCT339350.028.52394514
TTGTACT343100.028.3654521
CTAATGC340850.028.10493721
Produced by FastQC (version 0.11.5)