FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-2R-DREB2A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-2R-DREB2A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29312591
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT993810.3390386063108512No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT300910.1026555448476049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA256050.037.2700654
TTGTACT259150.036.2313161
ACTCTAG330850.035.518075
GTACTCT269850.035.2993743
TGTACTC275500.034.5628052
CTCTAGT296650.032.641316
TCTAGTT295600.032.5907947
CTAGTTG299600.031.3614678
ACCTACT265800.029.02790833
GGACACC324000.028.07228529
ACACCTA276350.028.00984231
ACCTCTA339700.027.6699317
GTTACCT340700.027.66056614
CTAATGC337250.027.62586621
GACACCT337500.027.22946530
TAGTTGT351550.027.1251779
TACCTCT349800.026.97108516
ATGCTGG346300.026.59346224
TGTTACC359300.026.29660613
TTACCTC359750.026.24443615
Produced by FastQC (version 0.11.5)