FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-2G-ABF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-2G-ABF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26463950
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1945830.7352757241454885No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT644640.24359175406543618TruSeq Adapter, Index 19 (97% over 37bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA531080.2006805484442043No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC405730.15331422557857008No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA383340.1448536594121437No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG336580.12718433945045998No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT289510.10939787900143402No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA282810.10686613298468293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG115150.050.2036811
TATGCCG103500.050.19630448
GTATGCC105400.049.8565747
CGTATGC105100.049.6657846
ACGTCTG122100.047.43221715
AGTCACG124050.045.4629128
CACACGT128650.045.09871312
GTCACGT125500.044.82945629
ACACGTC132050.044.0699413
AGCACAC132500.043.8678610
CACGTCT133000.043.59753414
TACTCTA431050.043.5231064
TCGTATG120450.043.42403845
CTCGTAT110650.042.9350144
GTACTCT439450.042.5952873
ACTCTAG504600.041.6177065
CACGTGA135500.041.494731
TCACGTG135800.041.3517930
ACGTGAT138800.040.5073932
TTGTACT458700.040.358211