Basic Statistics
Measure | Value |
---|---|
Filename | H7TFNBGXC_n01_0822-2G-ABF3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 26463950 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 194583 | 0.7352757241454885 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT | 64464 | 0.24359175406543618 | TruSeq Adapter, Index 19 (97% over 37bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 53108 | 0.2006805484442043 | No Hit |
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC | 40573 | 0.15331422557857008 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 38334 | 0.1448536594121437 | No Hit |
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG | 33658 | 0.12718433945045998 | No Hit |
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT | 28951 | 0.10939787900143402 | No Hit |
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA | 28281 | 0.10686613298468293 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACACG | 11515 | 0.0 | 50.20368 | 11 |
TATGCCG | 10350 | 0.0 | 50.196304 | 48 |
GTATGCC | 10540 | 0.0 | 49.85657 | 47 |
CGTATGC | 10510 | 0.0 | 49.66578 | 46 |
ACGTCTG | 12210 | 0.0 | 47.432217 | 15 |
AGTCACG | 12405 | 0.0 | 45.46291 | 28 |
CACACGT | 12865 | 0.0 | 45.098713 | 12 |
GTCACGT | 12550 | 0.0 | 44.829456 | 29 |
ACACGTC | 13205 | 0.0 | 44.06994 | 13 |
AGCACAC | 13250 | 0.0 | 43.86786 | 10 |
CACGTCT | 13300 | 0.0 | 43.597534 | 14 |
TACTCTA | 43105 | 0.0 | 43.523106 | 4 |
TCGTATG | 12045 | 0.0 | 43.424038 | 45 |
CTCGTAT | 11065 | 0.0 | 42.93501 | 44 |
GTACTCT | 43945 | 0.0 | 42.595287 | 3 |
ACTCTAG | 50460 | 0.0 | 41.617706 | 5 |
CACGTGA | 13550 | 0.0 | 41.4947 | 31 |
TCACGTG | 13580 | 0.0 | 41.35179 | 30 |
ACGTGAT | 13880 | 0.0 | 40.50739 | 32 |
TTGTACT | 45870 | 0.0 | 40.35821 | 1 |