FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-12R-FBH3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-12R-FBH3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23874706
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1268990.5315206813436781No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA382130.16005642121833877No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC270800.11342548050644059No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA266260.11152388640932374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT280000.043.1442071
TACTCTA298400.041.1750834
TGTACTC306900.039.8748782
ACTCTAG327150.039.589015
GTACTCT310450.039.509263
CTCTAGT339100.036.2642146
TCTAGTT341500.035.855047
CTAGTTG354300.034.0659078
ACCTCTA398700.030.25477217
TAGTTGT404050.030.2179289
ACCTACT321050.030.14826433
GGACACC390650.030.01202829
GTTACCT402650.029.8883214
CTAATGC399450.029.86042821
ACACCTA332050.029.29846831
TTACCTC412950.029.2020915
GACACCT401850.029.1750130
TACCTCT415550.028.97751416
CCTCTAA419900.028.71161718
ATGCTGG413650.028.6091324
Produced by FastQC (version 0.11.5)