FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-12G-GBF2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-12G-GBF2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28050428
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT4844381.727025341645411No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA2045590.7292544698426705No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG1525220.5437421489611495No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1129770.4027639079161287No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA944600.3367506549276182No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG726690.25906556577318535No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC712810.2541173346802409No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT674950.2406202144223967No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA575700.20523751010145014No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA496240.1769099565967407No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA492610.175615858695632No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG455470.16237541901321434No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG454180.16191553298224184No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT439400.15664645117001424No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT403540.143862332510577No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG395570.14102102114092518No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA362870.129363445006971No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA334520.11925664734955203No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA328940.11726737288999656No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC307890.10976303106676305No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG299690.10683972451329442No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT296070.10554919162017778No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC295760.10543867637242468TruSeq Adapter, Index 10 (97% over 36bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG283240.10097528636639698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT816450.055.369991
TGTACTC1011550.044.924782
GTACTCT1045350.043.5460743
TACTCTA1049750.043.4368134
ACTCTAG1058950.043.320515
GCACACG99950.043.3095111
CTCTAGT1097050.041.3571136
ACGTCTG108200.040.1694715
TCTAGTT1139600.039.766527
ACTCGGC104850.039.76533532
CACACGT112300.038.70256412
CACTCGG110500.038.1130731
GTATGCC70500.038.08714345
TATGCCG69350.038.01172646
CGGCAAT109200.037.40934435
ACACGTC116450.037.3535513
CTAGTTG1216050.037.0133938
AGCACAC118250.036.93292210
CGTATGC72900.036.25665344
CACGTCT122300.035.56706614
Produced by FastQC (version 0.11.5)