FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-11R-GBF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-11R-GBF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21890073
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1209450.5525107202703253No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA363420.16602046050737246No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA255630.1167789618609312No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC255420.11668302796431973No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT243510.11124220554221084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT261150.044.8116531
TGTACTC283650.041.4660382
TACTCTA287950.040.981164
GTACTCT295250.039.9793933
ACTCTAG348300.039.1955035
CTCTAGT323700.036.4662746
TCTAGTT330700.035.9473767
CTAGTTG350000.033.3852928
TAGTTGT385150.030.6379369
ACCTACT314650.030.0052333
ACCTCTA388600.029.76189617
GGACACC379200.029.72781829
ACACCTA320450.029.52944831
CTAATGC389600.029.5023421
GTTACCT391850.029.47046914
TTACCTC399250.029.03818115
GACACCT389350.029.02437430
TACCTCT403550.028.6594616
TGTTACC405200.028.63762513
ATGCTGG398650.028.4577324
Produced by FastQC (version 0.11.5)