FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-11G-HB7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-11G-HB7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20063685
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT765380.3814752873163629No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT297060.14805854457942297No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA215150.10723354159517556No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT205150.10224941230885552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA186700.038.948984
ACTCTAG252350.037.7467585
GTACTCT194750.037.267243
GCACACG42350.034.04393411
CTCTAGT216300.033.9751976
TCTAGTT216900.033.9294437
CTAGTTG218400.033.215728
GTATGCC39350.032.2952545
CGTATGC41100.031.1754544
TATGCCG41100.030.66399446
GGACACC235450.029.66369429
ACCTCTA245300.029.34541517
ACGTCTG48200.029.25891915
ACCTACT199600.029.21964633
GACACCT240200.029.12052730
GTTACCT248950.028.9992914
CTAATGC247300.028.8854221
ACACCTA205800.028.2893931
TTACCTC258550.027.88193515
ATGCTGG256750.027.74357624